Aphaenogaster ashmeadi is less often seen than other species in this genus. It is reported to have large colonies in the soil at the edges of forests and fields, and in sand under litter in hammocks, flatwoods, and longleaf pine forests. However, Joe MacGown has found this species to be somewhat elusive and has only collected it in sand hill habitats. If you look carefully you may be lucky enough to find this two-toned ant in your neighborhood.
Find out more about this species at Antweb.